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Techne转瓶(Spinner
Flask, Bibby-Scientific Ltd)中进行细胞培养,工作体积为60 ml。Spinner Flask转瓶以50
rpm搅拌混合。 微载体 Cytodex 3微载体被
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an equal amount of cells into the flasks. If five T-25 flasks were prepared, set micropipetter to 200 ml
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the cells using an inverted microscope to ensure that all the cells are detached and floating. The side of the flasks may
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所有原始文件打包,压缩率至少70%。用户可一键导入方法包文件,并将其改为只读模式(read only),防止误修改。 02采集数据 将导入的方法包文件复制到新建序列中,选择进样方法和处理方法后,即可采集数据。 03处理数据 如动画所示
2021-01-27
来源: 赛默飞色谱与质谱分析
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reported, but the full-length natures of only some have been determined. [provided by RefSeq, Dec 2008]
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of glass is cut, only further up the shaft ~200 um. This is cut by holding the shaft of glass on your forefinger with your
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生殖细胞发育不良(唯支持细胞综合征Sertoli Cell Only Syndrome,SCO):完全性生殖细胞发育不良的患者,睾丸曲细精管只含有sertoli细胞,没有精子生成的细胞。所以患者没有生育能力。在部分性生殖细胞发育不良
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:Trypsinize the cells from flasks, wash once in serum-free medium.Resuspend in room temperate serum-free medium
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应选择SIZE only。2)若样品为合金块状样品 ,结晶完整且加工过程中没有破碎,此时,线性宽化是由微观应变引起的,选择Strain only。3)如样品存在上述两种情况,则应选择size/strain。 三、注意a.利用XRD进行晶粒大小
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spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008]